Enhanced Heterotetrameric Assembly of Potato ADP-Glucose Pyrophosphorylase Using Reverse Genetics
نویسندگان
چکیده
منابع مشابه
Enhanced heterotetrameric assembly of potato ADP-glucose pyrophosphorylase using reverse genetics.
ADP-glucose pyrophosphorylase (AGPase) is a key allosteric enzyme in plant starch biosynthesis. Plant AGPase is a heterotetrameric enzyme that consists of large (LS) and small subunits (SS), which are encoded by two different genes. Computational and experimental studies have revealed that the heterotetrameric assembly of AGPase is thermodynamically weak. Modeling studies followed by the mutage...
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The potato tuber (Solanum tuberosum L.) ADP-glucose pyrophosphorylase activity is activated by a incubation with ADP-glucose and dithiothreitol or by ATP, glucose- 1-phosphate, Ca2+, and dithiothreitol. The activation was accompanied by the appearance of new sulfhydryl groups as determined with 5, 5'-dithiobis(2-nitrobenzoic acid). By analyzing the activated and nonactivated enzymes on SDS-poly...
متن کاملStructure Prediction of Potato Large Subunit ADP - Glucose Pyrophosphorylase
ADP-glucose pyrophosphorylase, a key enzyme involved in the biosynthesis of starch in higher plants, is composed of pairs of large (LS) and small subunits (SS). Its catalytic activity is activated by 3-phosphoglyceric acid (3-PGA) and inhibited by inorganic phosphate (Pi). Current evidence indicates that the two subunit types play distinct roles in enzyme function. The large subunit is involved...
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The potato tuber (Solanum tuberosum L.) ADP-glucose pyrophosphorylase (ADP-GlcPPase) catalyzes the first committed step in starch biosynthesis. The main type of regulation of this enzyme is allosteric, and its activity is controlled by the ratio of activator, 3-phosphoglycerate to inhibitor, P(i). It was reported (Fu, Y., Ballicora, M. A., Leykam, J. F., and Preiss, J. (1998) J. Biol. Chem. 273...
متن کاملCrystal structure of potato tuber ADP-glucose pyrophosphorylase.
ADP-glucose pyrophosphorylase catalyzes the first committed and rate-limiting step in starch biosynthesis in plants and glycogen biosynthesis in bacteria. It is the enzymatic site for regulation of storage polysaccharide accumulation in plants and bacteria, being allosterically activated or inhibited by metabolites of energy flux. We report the first atomic resolution structure of ADP-glucose p...
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ژورنال
عنوان ژورنال: Plant and Cell Physiology
سال: 2014
ISSN: 1471-9053,0032-0781
DOI: 10.1093/pcp/pcu078